Restriction enzyme mapping of vaccinia virus DNA

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran

The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22%...

Restriction mapping of retroviral vector episomal DNA.

The infidelity of reverse transcription enzyme activity during the replication cycle of a retrovirus can cause retroviruses to rapidly mutate. For gene therapy, this raises biosafety issues, such as the deletion of a therapeutic gene from a vector and the development of a replication-competent retrovirus. In a single retroviral replication cycle, a therapeutic herpes simplex virus thymidine kin...

DNA cloning without restriction enzyme and ligase.

One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing "customized cohesive ends" by a combination of PCR primer design and lambda exonuclease digestion. These complementary cohesive ends can form h...

A DNA nicking-closing enzyme encapsidated in vaccinia virus: partial purification and properties.

Vaccinia virus cores contain an activity which is able to relax both left-and right-handed superhelical DNA. This virus-specific nicking closing enzyme has been highly purified and differs from the corresponding host enzyme in salt optimum, in sedimentation coefficient, and in polypeptide composition as determined on sodium dodecyl sulfate/polyacrylamide gels. The enzyme is probably newly synth...